Table 8.

Wall clock time (s) and maximum RAM usage (GB) for algorithms on assigning long reads from ATCC MSA-1003 mock community

Algorithm	Time(s)	RAM(GB)
Mora	67.17	2.97
AugPatho2	15713.15	1.78
Minimap2	34712.47	23.87
Kraken2	522.15	76.79

Mora used fewer resources than AugPatho2 and was substantially faster. Time cannot be directly compared between the Mora/AugPatho2 and Kraken2 as they require the output from Minimap2. Bracken is not included as it is instantaneous once the Kraken2 result files are created. CPU time and other information can be found in Additional file 1: appendix Table A.4. All algorithms were run with 8 threads on a google c2-standard-60 machine type virtual machine