Extended Data Table 1.
Plasmids used in this study
| Use | Construct | Plasmid name | Plasmid backbone | Cloning method | Source |
|---|---|---|---|---|---|
| AvrSr13 | pDONR-AvrSr13 | pDONR207 | BP-Gateway | - | |
| AvrSr22 | pDONR-AvrSr22 | pDONR207 | BP-Gateway | - | |
| AvrSr22b | pDONR-AvrSr22b | pDONR207 | synthesis/BP-Gateway | - | |
| Sr13c | pDONR-Sr13c | pDONR207 | synthesis/BP-Gateway | - | |
| Sr22 | pDONR-Sr22 | pDONR207 | synthesis/BP-Gateway | - | |
| Expression vectors for wheat protoplasts | pUBQ-YFP | pTA22-YFP | pTA22 | - | - |
| pUBQ- empty vector | pTA22 | pTA22 | restriction digestion | ||
| pUBQ gateway cloning vector | pTA22-GW | pTA22 | restriction digestion | ||
| pUBQ gateway cloning vector with PBS | pTA22-GW-PBS | pTA22 | restriction digestion | ||
| pUBQ-RFP | pTA22-RFP | pTA22-GW | LR-Gateway | - | |
| pUBQ-Sr13c | pTA22-Sr13c | pTA22-GW | LR-Gateway | - | |
| pUBQ-Sr21 | pTA22-Sr21 | pTA22-GW | LR-Gateway | - | |
| pUBQ-Sr22 | pTA22-Sr22 | pTA22-GW | LR-Gateway | - | |
| pUBQ-Sr27 | pTA22-Sr27 | pTA22-GW | LR-Gateway | - | |
| pUBQ-Sr35 | pTA22-Sr35 | pTA22-GW | LR-Gateway | - | |
| pUBQ-Sr50 | pTA22-Sr50 | pTA22-GW | LR-Gateway | - | |
| pUBQ-Sr61 | pTA22-Sr61 | pTA22-GW | LR-Gateway | - | |
| pUBQ-AvrSr50 | pTA22-AvrSr50-PBS | pTA22-GW-PBS | LR-Gateway | - | |
| pUBQ-AvrSr27-2 | pTA22-AvrSr27-2-PBS | pTA22-GW-PBS | LR-Gateway | - | |
| pUBQ-AvrSr35 | pTA22-AvrSr35-PBS | pTA22-GW-PBS | LR-Gateway | - | |
| pUBQ-AvrSr13 | pTA22-0100-PBS | pTA22-GW-PBS | synthesis | ||
| pUBQ-AvrSr22 | pTA22-0336-PBS | pTA22-GW-PBS | synthesis | ||
| pUBQ-AvrSr2b | pTA22-AvrSr22b-PBS | pTA22-GW-PBS | LR-Gateway | - | |
| pUBQ-AvrSr2b | pTA22-AvrSr22b-PBS | pTA22-GW-PBS | LR-Gateway | - | |
| Expression vectors in Nicotiana | 35S-AvrSr50 | pBIN19-YFP-AvrSr50 | pBIN19-YFP-GTW | LR-Gateway | Chen et al.13 |
| 35S-AvrSr27-2 | pBIN19-YFP-AvrSr27-2 | pBIN19-YFP-GTW | LR-Gateway | Upadhyaya et al.11 | |
| 35S-Sr13c-YFP | pAM-Sr13c-YFP | pAM-GTW-YFP | LR-Gateway | - | |
| 35S-Sr22-YFP | pAM-Sr22-YFP | pAM-GTW-YFP | LR-Gateway | - | |
| 35S-YFP-AvrSr13 | pBIN19-YFP-AvrSr13 | pBIN19-YFP-GTW | LR-Gateway | - | |
| 35S-YFP-AvrSr22 | pBIN19-YFP-AvrSr22 | pBIN19-YFP-GTW | LR-Gateway | - | |
| 35S-YFP-AvrSr22b | pBIN19-YFP-AvrSr22b | pBIN19-YFP-GTW | LR-Gateway | - | |
| 35S-Sr27-YFP | pAM-Sr27-YFP | pAM-GTW-YFP | LR-Gateway | Upadhyaya et al.11 | |
| 35S-Sr50-YFP | pAM-Sr50-YFP | pAM-GTW-YFP | LR-Gateway | - |