FIG. 1.

Construction of two-hybrid fusion proteins. (A) Schematic diagram of the Tf1 proteins tested in the two-hybrid analysis. The coding sequences of the mature proteins are illustrated by solid vertical lines which represent predicted N-terminal and C-terminal PR processing sites. Dashed vertical lines represent subdomains used in the two-hybrid analysis. Sequence numbers on the line below represent base pairs starting from the 5′ LTR. The positions and names of the primers used in PCR amplification of various regions are indicated. (B) PCR primers used in the construction of BD and AD fusion proteins. Two independent bacterial transformants for both the BD and AD constructs were examined. The sequence is listed 5′ to 3′, and the restriction site in each primer is underlined. Thirteen additional nucleotides 5′ of the restriction site are not shown.