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. 2024 Apr 22;14:9181. doi: 10.1038/s41598-024-58845-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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CB1R-driven G protein activation induced by the tested cannabinoids. HEK293 cells were transiently transfected with G_αi1-Rluc8 or G_αoA-Rluc8, G_β2-Flag, G_γ2-VENUS, and SNAP-CB1R. The cells were stimulated with the indicated concentrations of (9S)-HHC, (9R)-HHC, Δ⁹-THC, WIN, or their vehicles. BRET donor and acceptor emission was measured 12 min after receptor stimulation. (A) Concentration–response relationship of G_αi1 subunit dissociation from the G protein complex after CB1R stimulation. (B) Concentration–response relationship of G_αoA subunit dissociation from the G protein complex after CB1R stimulation. The data are presented as means ± SEM from three independent experiments. The data analysis is disclosed in Supplementary Tables 1 and 2.