Figure 5.

ERK1/2 phosphorylation elicited by the cannabinoids. HEK293 cells were transiently transfected with CB1R and empty vector (1:2 ratio). 24 h after transfection, cells were stimulated by 10 µM of (9S)-HHC, (9R)-HHC, Δ⁹-THC or WIN, and kinetics of ERK1/2 phosphorylation were measured at the indicated times. The data are presented as means ± SEM of three independent experiments performed in 3 technical replicates. The statistical analysis is disclosed in Supplementary Table 6. *, p < 0.05 (9S)-HHC vs. WIN by ANOVA.