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. 2024 Apr 22;15:3389. doi: 10.1038/s41467-024-47691-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, c Contour plots showing PMA and Ionomycin (P/I)-induced IFNγ, IL-21, and IL-4 productions by TFH cells (as defined in supplementary Fig. 2d-f) in splenocytes from aged Mcpt8^DTR or Lyn^–/–Mcpt8^DTR mice basophil-depleted (DT + ) or not (DT–). Proportions (%) of IL-21+ (b) (n = 9/6/9/6), IL-4+ (d) (n = 9/4/9/4), IFNγ + (e) (n = 8/6/8/6) and IL-17A+ (n = 9/6/9/6) (f) TFH cells in splenocytes as in (a). g Contour plots of switched B cells (IgM^–IgD^–) among spleen CD45⁺TCRβ^–CD19⁺CD138^– cells in mice as in (a). h Proportions (%) of switched B cells among CD19⁺CD138^– splenocytes as in (g) (n = 6/6/7/8). i Contour plots of CD19⁺CD138⁺ cells among CD45⁺ cells from co-culture of sorted TFH cells from mice as in (g) and WT B cells. j Proportions (%) of CD19⁺CD138⁺ cells among CD45⁺ cells as in (i) (n = 6/7/5/4). k Contour plots of CD90.2⁺ and CD90.2^– TFH cells among spleen TFH cells from mice as in (a). Proportions (%) of CD90.2⁺ (l) and CD90.2^– (m) TFH cells among CD45⁺ splenocytes from mice as in (a) (n = 3/3/4/5). n Proportions (%) of CD90.2^– TFH cells among spleen TFH cells as in (k) from mice as in (g) (n = 3/3/4/5). CXCR5 expression on spleen TFH cells from mice as in (a–n) (o) and as in (t, u) (p). q Contour plots showing non-stimulated (–) and P/I-induced (+) IL-6 and IL-4 productions by spleen basophils from aged Mcpt8^DTR or Lyn^–/–Mcpt8^DTR mice. Proportions (%) of IL-6+ (r) (n = 8/8/8/8) and IL-4+ (s) (n = 11/11/11/11) basophils in splenocytes as in (a). Proportions (%) of IL-6 (t) (n = 9/9/8/8) and IL-4 (u) (n = 9/9/8/8) producing cells among basophils in splenocytes stimulated with P/I (+) or not (–) from PBS-injected Mcpt8^DTR (blue) or pristane-injected Mcpt8^DTR (red) mice. b, d–f, h, j, l–p, r–u Results are from at least three independent experiments and presented as individual values in bars representing the mean values ± s.e.m. Statistical analyses were by two-way ANOVA followed by Tukey’s multiple comparisons test (b, d–f, h, j, l–p) or by Kruskal–Wallis tests followed by two-sided Mann–Whitney U test between the indicated groups (r–u). P values are shown above each bracket. NS not significant. Source data are provided in the Source Data file.