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. 2024 Apr 22;15:3389. doi: 10.1038/s41467-024-47691-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Proportions (%) of TFH cells among CD4⁺ T cells in spleen (left) (n = 12/10/6/8) and lymph nodes (LN) (right) (n = 13/11/6/8) from Mcpt8^CT/+ (WT) (blue) or Mcpt8^CT/+Il4^fl/fl (Il4^fl/fl) (red) mice injected with PBS (–) or pristane (+). b Proportions (%) of basophils among CD45⁺ cells in the spleen (left) (n = 12/11/6/8) and lymph nodes (right) (n = 13/11/6/8) from mice as in (a). c Proportions (%) of CD19⁺CD138⁺ cells CD45⁺ cells in the spleen (left) (n = 11/11/6/8) and lymph nodes (right) (n = 12/10/6/8) from the mice described in (a). d Proportions (%) of spontaneous IL-6⁺ cells among basophils in the spleen from the mice described in (a) (n = 8/10/6/8). e Anti-RNP IgG plasma levels from the same mice as in (a) were quantified by ELISA and normalized to the mean of PBS-injected Mcpt8^CT/+ values (n = 9/9/5/7). (f) Quantification of C3 (left) and IgG (right) glomerular deposits in kidneys from the mice described in (a) (n = 10/9/6/8). A representative picture for each genotype in each condition is shown in Supplementary Fig. 9c, d. a–f The data shown that concerns Mcpt8^CT/+ (WT) mice are the same as the data shown in Fig. 6. g Anti-RNP IgM plasma levels were determined by ELISA and data were normalized to the mean of the PBS-injected control values for each genotype. The mice analyzed were Mcpt8^CT/+ (WT), Mcpt8^CT/+Il4^fl/fl (Il4^fl/fl), Mcpt8^CT/+Pdl1^fl/fl (Pdl1^fl/fl) (green) and basophil-deficient (Mcpt8^CT/+R26^DTA/+) (R26^DTA/+, gray) mice treated with PBS or pristane (– or +, respectively) (n = 13/12/5/8/4/6/6/5). h Representative pictures of kidneys from mice with the indicated genotypes injected with PBS or pristane showing the intensity of anti-IgM staining by immunofluorescence. Scale bar = 200 µm. i Quantification of IgM glomerular deposits in kidneys from the mice described in (g) (n = 15/15/6/8/4/6/5/5). a–i Results are from at least three independent experiments and presented as individual values in bars representing the mean values ± s.e.m. a–g, i Statistical analyses were done by two way ANOVA followed by Tukey’s multiple comparisons test between the indicated groups. P values are shown above each bracket. NS not significant. Source data are provided in the Source Data file.