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. 2024 Apr 22;14:9177. doi: 10.1038/s41598-024-59834-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Different in neuronal apoptosis in iPSCs-derived neuronal cell from GD patients. (a) Scatter plots represent a complete gating strategy in the flow cytometry analysis. (b) Scatter plots represent the intensities of PI and AnnexinV-FITC fluorescent at day 7, 10 and 14 of neuronal differentiation in control, GD3-1 and GD3-2 groups. (c) Bar graphs represent the percentage of apoptotic cells at day 7 (Kruskal–Wallis test: H(2) = 0.16, p = 0.95, n = 3 technical replicates), day 10 (Kruskal–Wallis test: H(2) = 5.97, p = 0.02 for Control vs. GD3-1, p = 0.46 for Control vs. GD3-2, n = 3 technical replicates) and day 14 (Kruskal–Wallis test: H(2) = 5.96, p = 0.02 for Control vs. GD3-1, p = 0.10 for Control vs. GD3-2, n = 3 technical replicates) of neuronal differentiation.