Table 2.
Sequence of the primers used for qPCR analysis.
| Gene | Forward (5′ -3′) | Reverse (5′ -3′) |
|---|---|---|
| RPL19 | AACTCCCGTCAGCAGATCC | AGTACCCTTCCGCTTACCG |
| PEDV M | TCCCGTTGATGAGGTGAT | AGGATGCTGAAAGCGAAAA |
| PEDV N | TTGGTGGTAATGTGGCTGTTC | TGGTTTCACGCTTGTTCTTCTT |
| PEDV S | CTCTCTGGTACAGGCAGCAC | GCTCACGTAGAGTCAAGGCA |
| IFABP | GAAACTTGCAGCTCATGACAAT | GTCTGCGAGGCTGTAGTTAAA |
| AREG | GAGTACGATAACGAACCGCACA | TTTCCACTTTTGCCTCCCTTT |
| MMP13 | AGTTTGGCCATTCCTTAGGTCTTG | GGCTTTTGCCAGTGTAGGTATAGAT |
| IL-1β | CAACGTGCAGTCTATGGAGT | GAGGTGCTGATGTACCAGTTG |
| REG3G | CTGTCTCAGGTCCAAGGTGAAG | CAAGGCATAGCAGTAGGAAGCA |
| IRF7 | CAGAAGCAGCTCCACTACAC | CTCCCAGTAGACTTTGCACTT |
| INOS | CTCCAGGTGCCCACGGGAAA | TGGGGATACACTCGCCCGCC |
| GSTO2 | GCCTTGAGATGTGGGAGAGAA | AAGATGGTGTTCTGATAGCCAAGA |
| NHE3 | AAGTACGTGAAGGCCAACATCTC | TTCTCCTTGACCTTGTTCTCGTC |
| TRPV6 | AGGAGCTGGTGAGCCTCAAGT | GGGGTCAGTTTGGTTGTTGG |
| APOA4 | ACCCAGCAGCTCAACACTCTC | GAGTCCTTGGTCAGGCGTTC |
| APOC3 | CTAACCAGCGTGAAGGAGTC | CAGAAGTCGGTGAACTTGCC |
Values are mean and pooled SEM, n = 6; RPL19: L19 ribosomal protein gene; PEDV M: membrane protein (porcine epidemic diarrhea virus); PEDV N: nucleocapsid protein (porcine epidemic diarrhea virus); PEDV S: spike protein (porcine epidemic diarrhea virus); IFABP: intestinal fatty acid binding protein; AREG: amphiregulin; MMP13: matrix metalloproteinase-13; IL-1β: nterleukin-1 beta; REG3G: regenerating islet-derived 3 gamma; IRF7: interferon regulatory factor 7; INOS: inducible nitric oxide synthase; GSTO2: glutathione-s-transferase omega 2; NHE3:Na(+)-H(+) exchanger 3; TRPV6: transient receptor potential vanilloid 6; APOA4: apolipoprotein A-IV; APOC3: apolipoprotein C3; a,b,c within a row, means with different superscripts differ, p < 0.05.