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. 2024 Apr 12;10(8):e29359. doi: 10.1016/j.heliyon.2024.e29359

Fig. 1.

Fig. 1

Cell composition of isolated OPC-enriched culture. a Scheme of the culture procedure. b Representative images of isolated cells (after 5 days of proliferation). Immunostaining for NG2 and Tuj1. P: PDGF, F: FGF, E: EGF. c Quantification of NG2+ OPC proportions after 5 days of proliferation according to media compositions corresponding to Table 1a (n = 3 biologically independent samples/experiments, 6 groups per sample, 5 images per group, approximately 300 DAPI + cells per image. One-way ANOVA and Tukey's post hoc test, two-sided. Data presented as mean values ± SEM.). d Quantification of Tuj1+ Neuron proportions after 5 days of proliferation according to media compositions corresponding to Table 1a (n = 3 biologically independent samples/experiments, 6 groups per sample, 5 images per group, approximately 300 DAPI + cells per image. One-way ANOVA and Tukey's post hoc test, two-sided. Data presented as mean values ± SEM.).