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. 2024 Apr 12;10(8):e29359. doi: 10.1016/j.heliyon.2024.e29359

Fig. 2.

Fig. 2

Proliferation of isolated primary OPC enriched culture. a Representative images of isolated cells (after 4.5 days of proliferation). Staining for NG2, EdU, Ki67. P: PDGF, F: FGF, E: EGF. b Quantification of Ki67+/DAPI + cells (%) according to media compositions corresponding to Table 1a (n = 3 biologically independent samples/experiments, 6 groups per sample, 10 images per group, approximately 500 DAPI + cells per image. One-way ANOVA. Data presented as mean values ± SEM.). c Quantification of EdU+/DAPI + cells (%) according to media compositions corresponding to Table 1a (n = 3 biologically independent samples/experiments, 6 groups per sample, 10 images per group, approximately 500 DAPI + cells per image. One-way ANOVA. Data presented as mean values ± SEM.). d Graph of WST-8 viability assay results, values relative to the DMEM (media composition 1) group cultured for 3 days (n = 3 biologically independent samples/experiments, 18 groups per sample, 3 replicates (wells) per group. One-way ANOVA and Tukey's post hoc test. Data presented as mean values ± SEM.).