Table 2.

Comparison of the E3 method with conventional primary OL culture methods.

Parameter	Method
	Shaking	Immunopanning	MACS	E3
Animal used	P1-4 rat	P6-8 rat/mouse	P5-8 rat/mouse	P1 rat/mouse
Dissection time (Sacrifice ∼ incubation)	Approx. 1.5 h 3min/pup dissection 30–60min dissociation	Approx. 4 h 3min/pup dissection 30–60min dissociation 15–45min panning*3	Approx. 2 h 3min/pup dissection 30–60min dissociation 35–45min MACS	Approx. 2 h 3min/pup dissection 30–60min dissociation 15min centrifuge*2
Time required for acquisition of OPCs	7–10 days Time until shaking	Approx. 4 h Dissection time	Approx. 2 h Dissection time	5–6 days Time until passage
Yield of OPCs	0.2–1*106/rat	2–2.5*106/rat 0.8–1*106/mouse	1*106/rat 0.37*106/mouse	3–5*106/rat 0.8–1*106/mouse
Purity of OPCs	90∼98 % Contaminant = Astrocytes, Microglia	>99.5 % Contaminant = Fibroblast-like cells	>90 %	98–99 % Contaminant = Neurons
Exclusive materials required	Fetal Bovine Serum Shaking Incubator	Antibodies (anti-RAN2, anti-BSL1, anti-GalC, anti-O4, anti-A2B5, anti-PDGFRα) Hybridomas	Anti-O4 antibody or microbeads Dissociation kit MACS dissociator Magnetic columns & separator	Iodixanol (OptiprepTM)
References	McCarthy & de Vellis, 1980 Yang et al., 2005 O'Meara et al., 2011 Zhu et al., 2014 Choi et al., 2017 Choi et al., 2023	Barres et al., 1992 Mayer-Proschel, 2001 Dugas & Emery, 2013a Dugas & Emery, 2013b Emery & Dugas, 2013	Dincman, Beare, Ohri, & Whittemore, 2012 Weil et al., 2019