Figure 6.

Effect of anti-CD40 antibody injection on human immune cell numbers and cytokine production in a human-stem-cell-transplanted mouse model. (a) Shown are changes in human immune cells in the peripheral blood of humanized mice injected with MAB 273, CP-870,893 or IgG1-LALA control antibodies as determined by flow cytometry. Bar graphs (mean + SD) with individual data points represent B-cell, T-cell and NK cell numbers as per cent of total human CD45+ cells in the peripheral blood of mice treated with the respective antibodies. Symbols depict individual mice. The statistical assessment of the data was performed with a one-way ANOVA test. * indicates a significant difference between the marked value and day 0. * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001. (b) The bar graphs (mean + SD) depict the concentration of human IL-12p70, TNF-α, IL-6 and IFN-γ at the indicated time points after the injection of MAB 273, CP-870,893 and IgG1-LALA control antibodies. Symbols indicate individual mice. Cytokine concentrations in murine serum were determined using a LEGENDplex^TM Multi-Analyte Flow Assay. Values between groups did not reach statistical significance as determined by a one-way ANOVA.