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Aflibercept produced in N. benthamiana. (A) Western blot analysis of total soluble proteins (TSP) extracted from plants infiltrated with pICH26211α-aflibercept (lane S, ~10 µg of TSP was loaded). (B) SEC profile of protein A-purified aflibercept and location of collected fractions. (C) SDS-PAGE of protein A-purified aflibercept (ELU), and SEC fractions thereof (F1–F3), under non-reducing (left) and reducing (right) conditions; protein loading (ELU, F1, F2, F3): 6.5, 2, 4, 4 µg/lane.
