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N-glycan composition of aflibercept expressed in N. benthamiana plants deficient in core xylose and fucose [18,24]. Four variants were analyzed by LC-ESI-MS: AFLIGnGn and AFLISia purified from TSP and IF. Histogram bars represent the relative abundance (%) of glycoforms present at each individual glycosite (GS1–GS5). For detailed information, see Table S1. Nomenclature according to Proglycan [28].
