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. 2024 Apr 22;12:64. doi: 10.1186/s40478-024-01769-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — a, b Heatmaps showing the top DEGs (top) and localizations of cell types or tumor cell states (bottom) in each of the eight annotated regions, cell type adjusted, for samples 18-0282 and 19-0142, respectively. Each column represents a spot in the corresponding region. If a gene is differentially expressed in multiple regions, it’s only shown once (See Additional file 21: Table S4 for the full DEGs)