Fig. 5.

Generation of IFNγ-secreting CD8⁺ T cells specific for TAA-derived peptides from peripheral blood of healthy donors. PBMCs from four healthy blood donors (III and VI) with undetectable levels of CD8⁺ T cells against epitopes from the TAAs: TPH-1, VMAT-1, and IA-2 were stimulated once with peptide-pulsed CD40L-matured DCs and three times with peptide-pulsed autologous blood cells. These CD8⁺ T cells reached IFNγ-secreting frequencies of 0.16–1.22% of the total CD3⁺CD8⁺ population, shown in the upper right quadrant. Background against C1R.A2 pulsed with an irrelevant HLA-A*0201-binding peptide are shown and have been subtracted from percentages indicated for relevant peptides