Fig 5.

A. hadrus-mediated biotransformation of α-fluoro-β-alanine into α-fluoro-β-ureidopropionic acid in vitro. (A–D) The extracted iron chromographs of 5-FU standard (A), FUPA standard (B), and the remaining 5-FU (C) and generated FUPA (D) after 1 h of biotransformation by A. hadrus; RT, retention time; m/z, mass-to-charge ratio under negative source (−H). (E–F) The consumption of 5-FU (E) and generation of FUPA (F) by A. hadrus. The equation shown in the panel was calculated by simple linear regression analyzed by GraphPad Prism 9.0. (G) The total molar concentration of 5-FU and FUPA in one system after biotransformation by A. hadrus at different times.