Fig. 3.

Wnt3a increases mitochondrial activity in CaV1.2 shRNA but decreasing activity in Tg737^orpk/orpk cells. a: Mitochondrial oxidative phosphorylation was used to indicate activity through Mito Tracker Red staining. Wnt3a was found to increase oxidative phosphorylation in scrambled and CaV1.2 shRNA cells while decreasing oxidative phosphorylation in Tg737^orpk/orpk when examined using fluorescence microscopy (bar = 20 μm). b: Results were quantified using flow cytometry. c: Two mitochondrial mRNAs encoded oxidative phosphorylation genes (CytC and ATP5γ1) were measured using PCR normalized to nuclear encoded β-actin (N = 3).