Fig. 4.

Wnt3a increases ROS and DNA damage in CaV1.2 shRNA but not in Tg737^orpk/orpk cells. a: Mitochondrial ROS was assessed by staining cells with MitoSOX. Wnt3a was found to increase ROS in scrambled and CaV1.2 shRNA cells while decreasing ROS in Tg737^orpk/orpk when examined using fluorescence microscopy (bar = 20 μm). b: Results were quantified using flow cytometry. c: Wnt3a increased formation of the oxidative DNA lesion 8-Oxoguanine in scrambled and CaV1.2 shRNA cells but had no effect on Tg737^orpk/orpk cells (N = 3).