Skip to main content
. Author manuscript; available in PMC: 2024 Apr 23.
Published in final edited form as: J Cell Physiol. 2014 Dec;229(12):1926–1934. doi: 10.1002/jcp.24642

Fig. 5.

Fig. 5.

Cilia modulates Wnt signaling to regulate CaV1.2 expression. Western blotting was performed on cellular protein extracts. Wnt3a treatment increased β-catenin accumulation in all cells, however, Tg737orpk/orpk cells displayed high basal levels of β-catenin. NF-κB p65 was blotted as a readout for DNA damage response (DDR) to ROS induced DNA lesions. Wnt3a induced NF-κB p65 expression in scrambled and CaV1.2 shRNA cells but decreased NF-κB p65 in Tg737orpk/orpk cells. CaV1.2 was overexpressed in Tg737orpk/orpk cells. Wnt3a treatment induced CaV1.2 expression in scrambled shRNA while decreasing expression in Tg737orpk/orpk cells. Data normalized to GAPDH for analysis (N = 3). Asterisks indicate significant difference from the corresponding control group (P < 0.05). # signs denote significant difference from the corresponding scrambled shRNA group (P < 0.05).