Figure 1.

Effect of cigarette smoke extract on RAW264.7 cells autophagy. RAW264.7 cells were treated with different concentrations of cigarette extract for 24 hours and 48 hours, and observed the effects of CSE concentrations and intervention time on the expression of autophagy related proteins (LC3B, P62, ATG5) in RAW264.7 cells. A, C, E and G: Changes in autophagy-related protein expression in RAW264.7 cells at different concentrations of cigarette smoke extract for 24 hours. B, D, F and H: Changes in autophagy-related protein expression in RAW264.7 cells at different concentrations of cigarette extracts for 48 hours