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CD147 coelutes with γ-secretase components during chromatographic purification. (A) In the Q-Sepharose HP chromatographic step, CD147, together with Psn-1 CTF and Nct, was found only on fractions eluting at a salt concentration of 200 mM. (Elution steps: 100 mM, 200 mM, 300 mM, 400 mM, 500 mM, and 1 M.) (B) In molecular sieve chromatography, CD147, Psn-1 CTF, and Nct coeluted at a molecular mass of 250–300 kDa.
