Fig 1. Schematics of bsAbs and neutralization activity against rVSV-EBOV.

(A) Schematic of bsAbs constructs, along with their nomenclature. The first designation in bsAb names reflects their format (“DV” for DVD-Ig, “SC” for scFv-IgG, “hSC” for hinge scFv-IgG, and “AS” for asymmetric). For symmetric bsAbs, Fvs with blue domains are the ones remaining in Fab format and their name appears second in order. For example, “DV_A774-A878” is a DVD-Ig format with A774 Fvs as the green (outer) domains and A878 Fvs as the blue (inner). (B) Heat map of neutralizing half-maximal inhibitory concentration (IC₅₀) values against rVSV-EBOV for the antibody panel. mAbs or bsAbs with curves that did not cross the 50% threshold are designated as non-neutralizing (NN).