Cell line (Homo-sapiens) |
HEK-293 |
ATCC |
CRL-1573 |
|
Cell line (Homo-sapiens) |
HEK-HCN4 |
Dr. Martin Biel; Zong et al., 2012
|
|
|
Cell line (Homo-sapiens) |
HEK-HCN4 |
This paper |
ATCC CRL-1573; pcDNA3.1 mHCN4 |
HEK-293 stably expressing HCN4 |
Cell line (Homo-sapiens) |
HEK-HCN2 |
This paper |
ATCC CRL-1573; pcDNA3.1 mHCN2 |
HEK-293 stably expressing HCN2 |
Cell line (Homo-sapiens) |
HEK-HCN4 Δ1–62 |
This paper |
ATCC CRL-1573; pTwist-CMV-WPRE-Neo mHCN4 Δ1–62 |
HEK-293 stably expressing HCN4 Δ1–62 |
Cell line (Homo-sapiens) |
HEK-HCN4 Δ1–130 |
This paper |
ATCC CRL-1573; pTwist-CMV-WPRE-Neo mHCN4 Δ1–130 |
HEK-293 stably expressing HCN4 Δ1–130 |
Cell line (Homo-sapiens) |
HEK-HCN4 Δ1–185 |
This paper |
ATCC CRL-1573; pTwist-CMV-WPRE-Neo mHCN4 Δ1–185 |
HEK-293 stably expressing HCN4 Δ1–185 |
Cell line (Homo-sapiens) |
HEK-HCN4 Δ1–200 |
This paper |
ATCC CRL-1573; pTwist-CMV-Hygro mHCN4 Δ1–200 |
HEK-293 stably expressing HCN4 Δ1–200 |
Cell line (Homo-sapiens) |
HEK-HCN4 PT/AF |
This paper |
ATCC CRL-1573; pcDNA3.1 mHCN4 PT/AF |
HEK-293 stably expressing HCN4 P545A/T547F |
Cell line (Homo-sapiens) |
HEK-HCN2 AF/PT |
This paper |
ATCC CRL-1573; pTwist-CMV-WPRE-Neo mHCN2 AF/PT |
HEK-293 stably expressing HCN2 A467P/F469T |
Recombinant DNA reagent |
pcDNA3.1 mHCN1 |
Dr. Eric Accili; Proenza et al., 2002
|
|
|
Recombinant DNA reagent |
pcDNA3.1 mHCN1 |
This paper; Liao et al., 2012
|
|
mHCN2 (sequence NP_032252.1) subcloned from pcDNA4 |
Recombinant DNA reagent |
pcDNA6 mHCN4 Δ1–25 |
Dr. Richard Aldrich; Liu and Aldrich, 2011
|
|
|
Recombinant DNA reagent |
pTwist-CMV-WPRE-Neo mHCN2 A467P/F469T |
This paper |
|
Synthesized by Twist Bioscience based on sequence NP_032252.1
|
Recombinant DNA reagent |
pTwist-CMV-WPRE-Neo mHCN4 |
This paper |
NP_001074661.1; codon optimized |
Synthesized by Twist Bioscience |
Recombinant DNA reagent |
pTwist-CMV-WPRE-Neo mHCN4 Δ1–62 |
This paper |
|
Deletions made using site-directed mutagenesis in pTwist-CMV-WPRE-Neo HCN4 |
Recombinant DNA reagent |
pTwist-CMV-WPRE-Neo mHCN4 Δ1–130 |
This paper |
|
Deletions made using site-directed mutagenesis in pTwist-CMV-WPRE-Neo HCN4 |
Recombinant DNA reagent |
pTwist-CMV-WPRE-Neo mHCN4 Δ1–185 |
This paper |
|
Deletions made using site-directed mutagenesis in pTwist-CMV-WPRE-Neo HCN4 |
Recombinant DNA reagent |
pTwist-CMV-Hygro mHCN4 Δ1–200 |
This paper |
|
Synthesized by Twist Bioscience based on sequence NP_001074661.1
|
Recombinant DNA reagent |
pcDNA3.1 mHCN4 P545T/A547F |
This paper |
|
Site-directed mutagenesis of pcDNA3.1 HCN4 by Applied Biological Materials |
Recombinant DNA reagent |
pcDNA3.1 mHCN4 V604X |
This paper |
|
Site-directed mutagenesis of pcDNA3.1 HCN4 by Applied Biological Materials |
Recombinant DNA reagent |
pcDNA3.1 mHCN4 S719X |
Proenza Lab; Liao et al., 2012
|
|
|
Recombinant DNA reagent |
pcDNA4 mHCN4-2 |
Proenza Lab; Liao et al., 2012
|
|
HCN4 residues 1–518 plus HCN2 residues 442–863 |
Recombinant DNA reagent |
pTwist-CMV-BG-WPRE-Neo mHCN2-4N VVGPT |
This paper |
|
Synthesized by Twist Bioscience based on sequence NP_001074661.1 and NP_032252.1
|
Recombinant DNA reagent |
pTwist-CMV-BG-WPRE-Neo mHCN2 VVGPT |
This paper |
|
Synthesized by Twist Bioscience based on sequence NP_032252.1
|
Recombinant DNA reagent |
pCMV6 Kan/Neo mLRMP |
Origene |
CAT#: MC201923
|
Untagged mouse LRMP construct |
Recombinant DNA reagent |
pCMV6 Kan/Neo Myc-mLRMP |
Proenza Lab; Peters et al., 2020
|
|
N-terminal Myc-tagged LRMP construct |
Recombinant DNA reagent |
pTwist-CMV mLRMP 1–227 |
This paper |
|
Synthesized by Twist Bioscience based on GenBank AAH52909.1
|
Recombinant DNA reagent |
pTwist-CMV mLRMP 228–539 |
This paper |
|
Synthesized by Twist Bioscience based on GenBank AAH52909.1
|
Recombinant DNA reagent |
pcDNA3.1 mHCN4 125-260Cer |
This paper |
|
C-terminal Cerulean; see DNA constructs section of the methods |
Recombinant DNA reagent |
pcDNA3.1 mHCN4 1-260Cer |
This paper |
|
C-terminal Cerulean; see DNA constructs section of the methods |
Recombinant DNA reagent |
pcDNA3.1 mHCN4 521-719Cer |
This paper |
|
C-terminal Cerulean; see DNA constructs section of the methods |
Recombinant DNA reagent |
pcDNA3.1 mHCN4 1-125Cer |
This paper |
|
C-terminal Cerulean; see DNA constructs section of the methods |
Recombinant DNA reagent |
pcMVBG mLRMP 1-479Cit |
This paper |
|
C-terminal Citrine; see DNA constructs section of the methods |
Recombinant DNA reagent |
pcMVBG mLRMP 1-230Cit |
This paper |
|
C-terminal Citrine; see DNA constructs section of the methods |
Recombinant DNA reagent |
pcMVBG mLRMP 1-108Cit |
This paper |
|
C-terminal Citrine; see DNA constructs section of the methods |
Recombinant DNA reagent |
pcMVBG mLRMP 110-230Cit |
This paper |
|
C-terminal Citrine; see DNA constructs section of the methods |
Commercial assay or kit |
Q5 Site-Directed Mutagenesis Kit |
New England Biolabs |
CAT#: E0554S |
|
Commercial assay or kit |
In-Fusion HD Cloning |
Clontech |
Clontech:639647 |
|
Chemical compound, drug |
FuGENE 6 |
Promega |
CAT#: E2691 |
|
Chemical compound, drug |
Lipofectamine 2000 |
Thermo-Fisher Scientific |
CAT#: 11668027 |
|
Software, Algorithm |
pClamp and clampfit |
Molecular Devices |
RRID:SCR_011323
|
|
Software, Algorithm |
ImageJ |
NIH DOI: https://doi.org/10.1038/nmeth.2089
|
RRID:SCR_003070
|
|
Software, Algorithm |
Sigmaplot 12.0 |
Systat Software Inc |
RRID:SCR_003210
|
|
Software, Algorithm |
JMP14 |
SAS Institute |
RRID:SCR_014242
|
|