FIG. 1.

trans activity is an authentic property of the DHBV polymerase. DHBV polymerase (expressed in yeast VLPs [TYDP], in permeabilized native viral cores [VP], or translated in vitro [DP]) was treated with micrococcal nuclease to degrade the endogenous nucleic acids and was employed in a trans assay with DRF⁺ RNA. The products were purified by phenol and chloroform extraction prior to resolution on a 6% denaturing polyacrylamide gel. DRF⁺ RNA, internally labeled DRF⁺ (264 nt); −YMHA, the polymerase active-site missense mutant. The mobilities of single-stranded RNA markers are indicated to the right.