FIG. 6.
The carboxy-terminal boundary of the ɛ-dependent papain-resistant fragment lies near amino acid 652. 35S-labeled DP and DP truncated at amino acid 728, 679, 652, or 657 were translated in vitro with or without ɛ (lanes 1 to 10) and were subjected to partial proteolysis with papain (lanes 11 to 20). The mobilities of the 89-kDa full-length DP and the 36-kDa protected fragment are indicated. DP derivatives were also employed in a priming assay (lanes 21 to 32) as described in the legend to Fig. 5.