FIG. 1.

Lytic transcription of vil-17. RNase protection analysis was performed to demonstrate vil-17 transcripts of wild-type herpesvirus saimiri C488. Protected fragments were observed in RNA samples from lytic cultures only. The signal was observed in virus-producing transformed C. jacchus P-1079 T cells (9) and in virus-infected OMK cells. Nonproductive human T cells (CB-15, CB-23) did not show this band, even after phorbol ester (tetradecanoyl phorbol acetate [TPA]) stimulation. The protected fragment of 217 nt corresponds to a transcription start site 52 nt upstream of the vIL-17 initiation codon. The probe template comprises 378 bp of viral sequence starting at the SpeI restriction site within the open reading frame and including 213 nt upstream of the vIL-17 ATG.