FIG. 3.

Northern blot analysis of progeny viral RNAs isolated from cucumber protoplasts inoculated with various combinations of viral RNA transcripts. The RNA transcripts used in the inoculations are indicated at the top, and the positions of the genome (gRNA), sg mRNAs (sgRNA1 and sgRNA2), and defective RNAs (DI-83) are shown at the left. The position of DI-83ΔMfeI is the same as that indicated for DI-83. Total nucleic acids were isolated and analyzed as described in the legend to Fig. 2.