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Northern blot analysis of progeny viral RNAs isolated from cucumber protoplasts inoculated with various combinations of viral RNA transcripts. The RNA transcripts used in the inoculations are indicated at the top, and the positions of the genome (gRNA), sg mRNAs (sgRNA1 and sgRNA2), and various defective RNAs are shown at the left. Total nucleic acids were isolated and analyzed as described in the legend to Fig. 2.
