FIG. 4.

Sequence of a vector-cellular junction identified by Alu-PCR from 293 cells transduced with the CMVβ-gal rAAV vector. (a) The vector portion of the junction includes the 5′ end of the CMV IE promoter (open box) and some AAV sequences (underlined) containing a partial AAV ITR. The cellular sequence contains a fragment with 64% homology to a human DNA sequence on chromosome Xq26.1 (dashed box), which is flanked by unidentified sequences not present in the CMVβ-gal vector (unmarked). The actual sequence in the shaded box is shown in panel b. The arrows in panels a and b point to the site of the vector-cellular junction.