FIG. 8.
B1-PCR analysis of rat brain samples. (a) Aliquots of 30 μl of B1-PCR products from 100 ng of genomic DNA were loaded on a 1% agarose gel containing ethidium bromide. Lanes 1 to 5, brain samples from a rat 2 weeks after injection with artificial CSF; lanes 6 to 10, samples from a rat injected with 5 × 105 IU of a crude preparation of the CMVβ-gal AAV vector; lanes 1 and 6, cerebral cortex; lanes 2 and 7, cerebellum; lanes 3 and 8, striatum; lanes 4 and 9, brainstem; lanes 5 and 10, hypothalamus. (b) The gel shown in panel a subjected to Southern blot analysis with a 32P-end-labeled specific CMV oligonucleotide probe (C3). (c and d) Alu-PCR amplification from 100 ng of hypothalamic genomic DNA isolated 2 weeks after injection with the CsCl gradient-purified CMVβ-gal. (c) Aliquots of 40 μl of PCR products were loaded in an agarose gel. Lane 1, B1-5-C1 at 10 pmol: 10 pmol; lane 2, C1 primer alone. (d) The gel shown in panel c subjected to Southern blot analysis.