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. 2024 Apr 23;7:491. doi: 10.1038/s42003-024-06077-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Dwell times were extracted using the f5c eventalign software. Each panel shows the delta in the mean dwell time values retrieved from the two experimental conditions (“Ribo”–“DNA only”) and separately calculated at each position of the forward (blue) and reverse (orange) strands. Each panel represents a subset of 11 positions on the forward (upper graphs) and reverse (bottom graphs) strands, centred on each genomic coordinate where single rNMPs were expected on the reverse strand: rA at position 4985 (a), rG at position 4997 (b), rC at position 5004 (c), and U at position 5015 (d) in “Ribo1A”; rG at position 4984 (e), U at position 4998 (f), and rA at position 5016 (g) in “Ribo1B”; rC at position 4996 (h) and rA at position 5007 (i) in “Ribo1C”. Dwell times are depicted in a 5′ to 3′ direction on the forward strand, so the complementary sequence is indicated for the reverse plots.