FIG. 4.

Corrected sensorgram overlays for the gDt-HveAt interaction. (A) Repeat injections of gD1 (306t) at the indicated concentrations are represented by the small symbols. Data were collected at 5 Hz, but for clarity, one point per second is shown. The solid lines represent the best fits found by the global software analysis. The inset represents a plot of k_obs versus concentration, the negative slope of which is equal to k_on. The R² for the linear fit of the data was 0.999. (B) Repeat injections of gD1 (Δ290-299t) at the indicated concentrations. Data collection and analysis were the same as described for panel A. The R² for the linear fit of the data in the plot of k_obs versus concentration (inset) was 0.999.