FIG. 7.

Binding of the cysteine variants to immobilized HveAt. (A) Binding of the gDt cysteine variants to HveAt by ELISA. An ELISA plate was coated with 200 nM HveAt in PBS, blocked, and incubated with various concentrations of gDt. Bound gDt was detected with antiserum R7 and then with peroxidase-conjugated secondary antibody and substrate. The data are averages of results from duplicate wells, and each experiment was repeated twice with similar results. Abs, absorbance. (B to D) Binding of gD1 (cys1,5), gD1 (cys2,6), and gD1 (cys3,4), respectively, to immobilized HveAt by SPR. Data are plotted and analyzed as described for Fig. 4.