Binding of the functional region variants to immobilized HveAt. (A) Binding of the gDt functional region variants to HveAt by ELISA. ELISA was done as described in the legend to Fig. 7. Abs, absorbance. (B to D) Binding of gD1 (∇34t), gD1 (∇126t), and gD1 (∇243t), respectively, to immobilized HveAt by SPR. Data are plotted and analyzed as described for Fig. 4.