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SDS-PAGE of two independent excystation experiments followed by labelling with the fluorescent dyes NHS-AF647 (red) and CFSE (green) of the different stages of centrifugation. (A) 1: lysate of 1×107oocysts; 2: lysate of sporozoite of 5 × 106 oocysts. (B) LEVs and (C) SEVs electrophoretic profiles, 1: sediment from 1×107oocysts; 2: sediment from 5 × 106 oocysts, respectively. Black arrows indicate common green bands; open arrows indicate red bands present only in the extract of SEVs.
