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. 2024 Apr 10;14:1367359. doi: 10.3389/fcimb.2024.1367359

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Copyright © 2024 Bertuccini, Boussadia, Salzano, Vanni, Passerò, Nocita, Scaloni, Sanchez, Sargiacomo, Fiani and Tosini

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunolocalization of CpRom1 by Western blotting on microvesicle extracts and immunoelectron microscopy of the corresponding extracellular vesicles. (A) Immunoblotting with anti-CpRom1 mouse serum: 1, unexcysted oocysts lysate; 2, excysted soporozoites; 3, LEVs extract; 4, SEVs extract; 5, TCA-precipitated supernatant of SEVs. (B) Negative staining immunoelectron microscopy of LEVs labelled with anti-CpRom1 rabbit serum. (C) Negative staining immunoelectron microscopy of LEVs labelled with pre-immune rabbit serum. (D) Negative staining immunoelectron microscopy of SEVs labelled with anti-CpRom1 rabbit serum.