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. 2024 Apr 10;14:1367359. doi: 10.3389/fcimb.2024.1367359

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Copyright © 2024 Bertuccini, Boussadia, Salzano, Vanni, Passerò, Nocita, Scaloni, Sanchez, Sargiacomo, Fiani and Tosini

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Physical characterization of LEVs and SEVs by electron-microscopy and nanoparticle tracking analysis (NTA). (A) TEM negative staining of LEVs. (B) TEM negative staining of SEVs. (C) graph showing the vesicle size distribution in LEVs (green) and SEVs (orange). Statistical analysis was based on TEM micrographs; dots indicate maximum and minimum values (****=p-value<0.0001, Mann-Whitney test). (D) graph showing the size distribution of LEVs as dtermined by NTA. (E) graph showing the distribution of SEVs as determined by NTA. (F) table reporting the mode diameter, the concentration of LEVs and SEVs, the total number of EVs (LEVs or SEVs) in 0.5 ml as determined by NTA.