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Western blotting experiments on oocyst-sporozoite lysates and EVs probed with mouse anti-CpGRASP serum. 1, lysate oocyst-sporozoite probed with pre-immune serum; 2, lysate oocyst-sporozoite; 3, LEVs extract; 4, SEVs extract; 5, SEVs supernatant precipitated with TCA. 4-20% SDS-PAGE, lane 1 probed with 1:500 with mouse serum before the immunization; lane 2-5 probed with 1:500 mouse serum after the immunization with recombinant CpGRASP.
