Table 1.
List of identified proteins in C. parvum EVs by proteomic experiments.
| Protein ID (CryptoDB) | Gene product (CryptoDB) | MW (kDa) | GO terms (CryptoDB) -Presumptive function and/or localization | EVs DB[1] | LEVs | SEVs |
|---|---|---|---|---|---|---|
| cgd1 3020-RA | Fructose-bisphosphate aldolase | 38.5 | glycolytic process | √ | y | y |
| cgd1 3040-RA | Triosephosphate isomerase | 27.4 | glycolytic process gluconeogenesis | √ | y | y |
| cgd1 870-RA | Peptidyl-prolyl cis-trans isomerase | 22.9 | protein peptidyl-prolyl isomerization/protein folding | √ | y | y |
| cgd2 20-RA | Heat shock-70 protein | 73.3 | No Data | √ | y | y |
| cgd2 3330-RA | Hsp70-protein | 103 | ATP binding | √ | y | y |
| cgd2 3950-RA | Translation-elongation factor EF1B/ribosomal protein S6 | 27.6 | translational elongation | √ | y | y |
| cgd2 4320-RA | Thioredoxin/glutathione reductase selenoprotein | 56.3 | cell redox homeostasis | √ | y | y |
| cgd3 1290-RA | 14-3 -3 domain containing protein | 28.6 | No Data | √ | y | y |
| cgd3 1540-RA | Signal peptide containing protein | 185 | No Data | √ | y | y |
| cgd3 2090-RA | 40S ribosomal protein Sae | 28.3 | Translation/Ribosome | √ | y | y |
| cgd3 3370-RA | Uncharacterized protein | 166 | No Data | √ | y | y |
| cgd3 3430-RA | Amine oxidase | 199 | amine metabolic process/copper ion binding | √ | y | y |
| cgd3 3770-RA | Hsp90 protein | 80.8 | protein folding/ATP binding | √ | y | y |
| cgd4 1940-RA | Nucleoside diphosphate kinase | 16.8 | nucleoside diphosphate phosphorylation | √ | y | y |
| cgd4 2600-RA | UDP-glucose 4-epimerase | 38.5 | galactose metabolic process/UDP-glucose 4-epimerase activity | √ | y | y |
| cgd4 3270-RA | Hsp70 protein | 90.7 | ATP binding | √ | y | y |
| cgd4 740-RA | Thioredoxin peroxidase-like protein | 21.8 | peroxidase activity | √ | y | y |
| cgd5 1960-RA | Enolase | 48.4 | glycolytic process/magnesium ion binding | √ | y | y |
| cgd5 2020-RA | Cysteine-rich secretory protein. Allergen V5/Tpx-1-related | 46.2 | No Data | √ | y | y |
| cgd5 2800-RA | Actin depolymerizing factor | 15.5 | actin filament depolymerization/actin cytoskeleton/actin binding | √ | y | y |
| cgd5 3160-RA | Actin | 42.1 | No Data | √ | y | y |
| cgd5 3360-RA | Adenylate kinase | 24.2 | adenylate kinase activity | √ | y | y |
| cgd5 3740-RA | 50S ribosomal protein L7e/L30e/S12e/Gadd45 | 15.8 | translation/Ribosome | √ | y | y |
| cgd6 1080-RA | Glycoprotein GP40 | 33.4 | No Data | √ | y | y |
| cgd6 120-RA | Disulfide-isomerase. Signal peptide plus ER retention motif | 53.8 | endoplasmic reticulum lumen/protein disulfide isomerase activity | √ | y | y |
| cgd6 2450-RA | Glycogen/starch/alpha-glucan phosphorylase | 104 | carbohydrate metabolic process/glycogen phosphorylase activity | √ | y | y |
| cgd6 2690-RA | FKBP-like peptidyl-prolyl isomerase | 36.8 | protein folding/peptidyl-prolyl cis-trans isomerase activity | √ | y | y |
| cgd6 3790-RA | Glyceraldehyde-3-phosphate dehydrogenase | 36.1 | glycolytic process/glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity | √ | y | y |
| cgd6 4460-RA | Uncharacterized protein with Armadillo-like helical | 276 | No Data | √ | y | y |
| cgd6 710-RA | Uncharacterized Secreted Protein | 49.7 | No Data | √ | y | y |
| cgd7 1710-RA | Threonyl-tRNA synthetase | 87.6 | tRNA aminoacylation/cytoplasm/aminoacyl-tRNA ligase activity | √ | y | y |
| cgd7 3670-RA | Heat shock protein Hsp90 | 89.1 | protein folding/ATP binding | √ | y | y |
| cgd7 400-RA | Uncharacterized protein | 38.6 | No Data | √ | y | y |
| cgd7 4310-RA | Cysteine-rich secretory protein. Allergen V5/Tpx-1-related | 185 | No Data | √ | y | y |
| cgd7 4450-RA | Elongation factor EF1-gamma (Glutathione S-transferase family) | 43.1 | translational elongation/transferase activity | √ | y | y |
| cgd7 480-RA | L-lactate/malate dehydrogenase | 33.9 | carbohydrate metabolic process/oxidoreductase activity | √ | y | y |
| cgd7 910-RA | Phosphoglycerate kinase | 42 | glycolytic process/phosphoglycerate kinase activity | √ | y | y |
| cgd8 1720-RA | Aldehyde/Alcohol dehydrogenase | 89.7 | alcohol dehydrogenase (NAD+) activity | √ | y | y |
| cgd8 2930-RA | Gtpase translation elongation factor 2 | 92.7 | GTPase activity | √ | y | y |
| cgd1 2040-RA | Pyruvate kinase | 56.4 | glycolytic process/pyruvate kinase activity | √ | y | n |
| cgd1 660-RA | Signal peptide region containing protein | 37.1 | No Data | √ | y | n |
| cgd4 2300-RA | Ubiquitin-activating enzyme E1 | 120 | cellular protein modification process/ubiquitin-like modifier activating enzyme activity | √ | y | n |
| cgd7 2280-RA | Ribosomal protein L40e | 14.7 | translation/ribosome/structural constituent of ribosome | √ | y | n |
| cgd7 4270-RA | 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase | 28.3 | glycolytic process/phosphoglycerate mutase activity | √ | y | n |
| cgd1 2860-RA | UbiE/COQ5 methyltransferase | 32.4 | methylation/methyltransferase activity | √ | n | y |
| cgd1 3690-RA | Aspartyl (Acid) protease | 88.4 | proteolysis/integral component of membrane/aspartic-type endopeptidase activity | √ | n | y |
| cgd2 3780-RA | Uncharacterized protein | 58.9 | No Data | √ | n | y |
| cgd2 4120-RA | Peptidyl-prolyl cis-trans isomerase | 18.5 | protein folding/peptidyl-prolyl cis-trans isomerase activity | √ | n | y |
| cgd2 860-RA | Proteasome subunit beta type | 22.9 | proteolysis involved in cellular protein catabolic process/proteasome core complex/threonine-type endopeptidase activity | √ | n | y |
| cgd5 1650-RA | DJ-1/PfpI | 19.6 | No Data | √ | n | y |
| cgd5 3230-RA | Manganese/iron superoxide dismutase | 25.5 | superoxide metabolic process/superoxide dismutase activity | √ | n | y |
| cgd5 440-RA | Adenylate cyclase-associated CAP | 19 | cytoskeleton organization/actin binding | √ | n | y |
| cgd6 1630-RA | CS domain containing protein | 21.2 | No Data | √ | n | y |
| cgd6 3180-RA | 40S ribosomal protein S15 | 16.4 | translation/ribosome/small ribosomal subunit/structural constituent of ribosome | √ | n | y |
| cgd6 3970-RA | Glutaredoxin-like protein 2 thioredoxin folds | 24.7 | protein disulfide oxidoreductase activity/iron-sulfur cluster binding | √ | n | y |
| cgd6 4320-RA | 40S ribosomal protein S5 | 21.8 | translation/small ribosomal subunit/structural constituent of ribosome | √ | n | y |
| cgd7 220-RA | GTP-binding nuclear protein | 24.1 | nucleocytoplasmic transport/nucleus/GTPase activity | √ | n | y |
| cgd7 3120-RA | Thiamine pyrophosphate enzyme | 64.4 | carboxy-lyase activity/thiamine pyrophosphate binding | √ | n | y |
| cgd7 360-RA | Heat shock protein 70 | 71.8 | endoplasmic reticulum/ATP binding | √ | n | y |
| cgd8 2110-RA | MIR motif-containing 39-like glycosyltransferase | 25.4 | membrane | √ | n | y |
[1]Presence of related proteins in Vesiclepedia (http://microvesicles.org) or ExoCarta (http://exocarta.org): √
Proteins in the light blue background were found both in LEVs and SEVs, proteins in yellow background were found only in LEVs, and proteins in green background only in SEVs.