Figure 8.

MBD interaction with H3-derived peptides by ITC. Calorimetric titrations of MBD interacting with H3-derived peptides: wild-type and trimethylated at K27. The upper panels show the thermograms (thermal power as a function of time to maintain the same temperature in the sample cell with respect to the reference cell), and the lower panels show the binding isotherms (ligand-normalized heat effect per injection as a function of the molar ratio in the sample cell). The continuous lines correspond to the non-linear least-squares fitting according to a single binding site model.