Table 3.

MBD interacts with the lysine-trimethylated H3 variants

		K a (M−1)	K d (μM)	ΔH (kcal/mol)
MBD	H3	2.0 × 107	0.050	–19.0
	H3 K4me3	8.4 × 106	0.12	–4.1
	H3 K9me3	1.3 × 107	0.075	–59.0
	H3 K27me3	1.7 × 105	5.9	7.0
	H3 K36me3	3.3 × 105	3.1	4.0
MBD R106W	H3	1.9 × 107	0.052	–18.1
	H3 K4me3	3.2 × 106	0.32	–15.5
	H3 K9me3	2.0 × 107	0.051	–51.9
	H3 K27me3	1.6 × 105	6.2	15.3
	H3 K36me3	6.1 × 104	16	7.7
MBD R133C	H3	2.5 × 107	0.040	–26.2
	H3 K4me3	3.5 × 106	0.29	–12.8
	H3 K9me3	2.2 × 107	0.046	–51.1
	H3 K27me3	1.3 × 105	7.9	10.7
	H3 K36me3	6.7 × 105	1.5	–3.7

Binding parameters for MBD (WT and Rett-associated variants) interacting with H3 trimethylated at K4, K9, K27 and K36 at pH 7, 20°C.