Figure 7.

Reconstruction of the cascade of gRNAs required to direct editing of the pan-edited A6 and RPS12 cryptogenes. The top line in each panel represents the cryptogene DNA sequence. Under the DNA line, the gRNA sequences (in 3′-5′ direction) are displayed, aligned to the corresponding positions of the mature mRNA. Inserted and deleted nucleotides are shown in light-red lowercase (u) and blue (T), respectively. Vertical lines ‘|’, colons ‘:’ and hashes ‘#’ in alignments represent Watson–Crick pairs, G:U pairs and other mRNA:gRNA interactions, respectively. The label ‘mcN’ refers to an aligned gRNA, where N is the number of minicircle encoding a particular gRNA (Supplementary Table S4). The encoded amino acid sequence is presented under the row of mature mRNA (at the bottom of each panel).