Figure 5.
ES7S ablation alters ribosomal dynamics. Cells were grown in the presence of different concentrations of antibiotics and the reducing agent DTT. Growth curves were recorded and based on the doubling times for each concentration, IC50 was calculated. The reagents used were (A) DTT, (B) anisomycin, (C) geneticin, (D) paromomycin, (E) cycloheximide and (F) blasticidin S. Cells were treated with 110 mM DMS for 5 min and total RNA isolated. RT assays were performed to test the dynamics of the A-site finger (G, H) and helix 84 (I, J). Lines indicate (G) the tip of the A-site finger. Arrowheads in G indicate the DMS reactive nucleotides C982 and C961 and in I the residues C2680 and C2675 and the asterisk indicates the DMS independent band used for normalization. The # in I represents the nucleotide A2678.