Figure 5.

Assessment of the strength of the identified MKHK-TAZ2 methylation profile and cross-validation

(A) Hierarchical clustering, with red representing TAZ2 case samples and blue depicting matched control samples in the heatmap panel.

(B) MDS, where red and blue circles represent case and control samples, respectively. A mild difference is observed between the methylation patterns of the case and control groups in both plots (A and B).

(C) Cross-validation results for the TAZ2 episignature. In six iterations, the TAZ2 sample that was not used for probe selection, indicated with black, clustered with control individuals, demonstrated by blue circles (C.T.18, C.T.1, C.T.8, C.T.2, C.T.7, and C.T.25) and, in five rounds, the testing TAZ2 sample clustered between the training TAZ2 samples, red circles, and the control individuals (C.T.13, C.T.10, C.T.5, C.T.12, C.T.4), indicating that the selected probes are not sensitive enough to classify all TAZ2 samples correctly.