Figure 3.
On day 21 after injury, the effects of BA on neuronal apoptosis and neuron damage in SCI rats were observed.
(A, B) The degree of apoptosis in the spinal cord of SCI rats was measured by TUNEL staining (original magnification 400×). The green fluorescence signal was strongest in the model group. BA and methylprednisolone treatment decreased the green fluorescence intensity. Scale bars: 50 μm. (C, D) The number of Nissl bodies in the anterior horn of the spinal cord in SCI rats was determined by Nissl staining (original magnification 400×). The model group exhibited fewer Nissl bodies than the sham group. BA and methylprednisolone treatment increased the number of Nissl bodies. Scale bars: 50 μm. Data are expressed as mean ± SD (n = 6). **P < 0.01, vs. sham group; ##P < 0.01, vs. model group (one-way analysis of variance followed by Tukey's post hoc test). BA: Biochanin A; PC: positive control; SCI: spinal cord injury; TUNEL: terminal deoxynucleotidyl transferase dUTP nick end labeling.