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. 2023 Dec 15;19(9):2050–2056. doi: 10.4103/1673-5374.390953

Figure 7.

Figure 7

BA inhibits apoptosis and oxidative stress and promotes autophagy in the spinal cord tissue of SCI rats.

(A) Western blot analysis of apoptosis-related protein expression levels. (B–D) Quantification of cleaved-caspase-3 (B), Bax (C), and Bcl-2 (D) protein levels from the western blot data. (E) Western blot analysis of autophagy-related protein expression levels. (F–H) Quantification of LC3II/LC3I (F), Beclin-1 (G), and P62 (H) protein levels from the western blot data. (I) Western blot analysis of oxidative stress-related protein expression levels. β-Actin was used as an internal reference for cleaved-caspase-3, Bax, and Bcl-2. GAPDH was used as an internal reference for LC3I, LC3II, Beclin-1, P62, Nrf2, and HO-1. (J, K) Western blot analysis of Nrf2 (J) and HO-1 (K) protein expression levels. All western blot data were normalized to the sham group. Data are expressed as mean ± SD (n = 3). **P < 0.01, vs. sham group; #P < 0.05, ##P < 0.01, vs. model group (one-way analysis of variance followed by Tukey's post hoc test). BA: Biochanin A; HO-1: heme oxygenase-1; LC3: microtube-associated protein 1 light-chain 3; Nrf2: nuclear factor erythroid2-related factor 2; P62: sequestosome-1; PC: positive control; SCI: spinal cord injury.