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. 2023 Dec 15;19(9):2027–2035. doi: 10.4103/1673-5374.390952

Figure 3.

Figure 3

Neurological evaluations of rats at 1 month after hpcMSCs transplantation.

(A) Immunofluorescence staining of hpcMSCs showed that the cells expressed CD90+/CD44+CD45−, consistent with mesenchymal stem cell characteristics. The green (Dylight 488) indicates CD90- or CD44-positive cells, red (Dylight 594) indicates CD45-positive cells, and blue represents DAPI (nuclei). Scale bars: 50 μm. (B) Quantification diagrams for number of grooming times, time spent grooming, number of feces, number of rearing times, and time spent rearing in the open field test. (C) Quantification diagrams for latency to target, number of target crossings, and distance traveled in target quadrant in the Morris water maze test. (D) The quantification diagrams for time spent in food arm, the number of food arm entries, accuracy, time spent in error arm, number of error arm entries, and the error rate in the Y-maze test. (E) The duration in the rotarod test. (F) The neurological severity scores. All data are presented as mean ± SD (n = 5/group). *P < 0.05 (one-way analysis of variance followed by Tukey's post hoc test). AL: Adjacent left quadrant; AR: adjacent right quadrant; DAPI: 4′,6-diamidino-2-phenylindole; HI: hypoxia-ischemia; hpcMSCs: human placental chorionic derived mesenchymal stem cells; NS: normal saline; O: opposite quadrant; T: target quadrant.