Figure 5.

C1q/tumor necrosis factor-related protein-6 (CTRP6) overexpression attenuates ischemia/reperfusion (I/R)-induced inflammatory responses and apoptosis in the brain.

(A–D) mRNA levels of inflammatory factors (A), interleukin-1β (IL-1β) protein level (B), tumor necrosis factor-α (TNF-α) protein level (C), and monocyte chemoattractant protein-1 (MCP-1) protein level (D) were measured to estimate inflammatory responses caused by 24-hour I/R (six animals per group). (E) Nuclear factor kappa B (NF-κB) signaling pathway (six animals per group). (F) Protein expression levels of Bcl-2 and Bax (six animals per group). (G, H) Caspase-3 and caspase-9 activity (six animals per group). (I) TUNEL staining in the brain (six animals per group). Blue is positive signal as indicated in the I/R group. Scale bar: 100 μm. All data were normalized to the Sham group. The results are presented as the mean ± SEM, and were analyzed by one-way analysis of variance followed by post hoc Tukey's test. *P < 0.05, vs. Sham group; #P < 0.05, vs. I/R group. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; TUNEL: terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling.